Document Detail


The small subunit processome is required for cell cycle progression at G1.
MedLine Citation:
PMID:  15356263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Without ribosome biogenesis, translation of mRNA into protein ceases and cellular growth stops. We asked whether ribosome biogenesis is cell cycle regulated in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, and we determined that it is not regulated in the same manner as in metazoan cells. We therefore turned our attention to cellular sensors that relay cell size information via ribosome biogenesis. Our results indicate that the small subunit (SSU) processome, a complex consisting of 40 proteins and the U3 small nucleolar RNA necessary for ribosome biogenesis, is not mitotically regulated. Furthermore, Nan1/Utp17, an SSU processome protein, does not provide a link between ribosome biogenesis and cell growth. However, when individual SSU processome proteins are depleted, cells arrest in the G1 phase of the cell cycle. This arrest was further supported by the lack of staining for proteins expressed in post-G1. Similarly, synchronized cells depleted of SSU processome proteins did not enter G2. This suggests that when ribosomes are no longer made, the cells stall in the G1. Therefore, yeast cells must grow to a critical size, which is dependent upon having a sufficient number of ribosomes during the G1 phase of the cell cycle, before cell division can occur.
Authors:
Kara A Bernstein; Susan J Baserga
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-09-08
Journal Detail:
Title:  Molecular biology of the cell     Volume:  15     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-26     Completed Date:  2005-04-08     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5038-46     Citation Subset:  IM    
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle*
Flow Cytometry
Fungal Proteins / chemistry
G1 Phase*
G2 Phase
Immunoprecipitation
Microscopy, Fluorescence
RNA, Messenger / metabolism
RNA, Ribosomal / chemistry
RNA, Small Nucleolar / metabolism
Ribonucleoproteins, Small Nucleolar / chemistry*,  physiology*
Ribosomes / chemistry*,  metabolism
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / chemistry*,  physiology*
Schizosaccharomyces / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
CA-16359/CA/NCI NIH HHS; F31 GM067564/GM/NIGMS NIH HHS; GM-07499/GM/NIGMS NIH HHS; GM-52581/GM/NIGMS NIH HHS; GM-67564/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Nan1 protein, S cerevisiae; 0/RNA, Messenger; 0/RNA, Ribosomal; 0/RNA, Small Nucleolar; 0/RNA, U3 small nucleolar; 0/Ribonucleoproteins, Small Nucleolar; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

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