Document Detail


The small nucleoid protein Fis is involved in Vibrio cholerae quorum sensing.
MedLine Citation:
PMID:  17181781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quorum sensing is a process of cell-cell communication that bacteria use to relay information to one another about the cell density and species composition of the bacterial community. Quorum sensing involves the production, secretion and population-wide detection of small signalling molecules called autoinducers. This process allows bacteria to synchronize group behaviours and act as multicellular units. The human pathogen, Vibrio cholerae, uses quorum sensing to co-ordinate such complex behaviours as pathogenicity and biofilm formation. The quorum-sensing circuit of V. cholerae consists of two autoinducer/sensor systems, CAI-1/CqsS and AI-2/LuxPQ, and the VarS/A-CsrA/BCD growth-phase regulatory system. Genetic analysis suggests that an additional regulatory arm involved in quorum sensing exists in V. cholerae. All of these systems channel information into the histidine phosphotransfer protein, LuxU, and/or the response regulator, LuxO. LuxO, when phosphorylated, activates the expression of four genes encoding the Qrr (quorum regulatory RNAs) small RNAs (sRNAs). The Qrr sRNAs destabilize the hapR transcript encoding the master regulator of quorum sensing, HapR. Here we identify the nucleoid protein Fis as playing a major role in the V. cholerae quorum-sensing circuit. Fis fulfils the predictions required to be the putative additional component that inputs information into the cascade: its expression is regulated in a growth phase-dependent manner; it requires LuxO but acts independently of LuxU, and it regulates all four qrr genes and, in turn, HapR by directly binding to the qrr gene promoters and modulating their expression.
Authors:
Derrick H Lenz; Bonnie L Bassler
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-12-20
Journal Detail:
Title:  Molecular microbiology     Volume:  63     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-16     Completed Date:  2007-06-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  859-71     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA.
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MeSH Terms
Descriptor/Qualifier:
Factor For Inversion Stimulation Protein / metabolism*
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Promoter Regions, Genetic
Quorum Sensing*
RNA, Bacterial / genetics
Repressor Proteins / metabolism
Signal Transduction
Vibrio cholerae / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
5R01 G065859//PHS HHS
Chemical
Reg. No./Substance:
0/Factor For Inversion Stimulation Protein; 0/RNA, Bacterial; 0/Repressor Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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