Document Detail

A small heat shock protein is essential for thermotolerance and intracellular survival of Leishmania donovani.
MedLine Citation:
PMID:  25179594     Owner:  NLM     Status:  In-Data-Review    
Leishmania parasites must survive and proliferate in two vastly different environments - the guts of poikilothermic sandflies and the antigen-presenting cells of homeothermic mammals. The change of temperature during the transmission from sandflies to mammals is both a key trigger for the progression of their life cycle and for elevated synthesis of heat shock proteins, which have been implicated in their survival at higher temperatures. Although the functions of the main heat shock protein families in the Leishmania life cycle have been studied, nothing is known about the roles played by small heat shock proteins. Here, we present the first evidence for the pivotal role played by the Leishmania donovani 23-kDa heat shock protein (which we called HSP23), which is expressed preferentially during the mammalian stage where it assumes a perinuclear localisation. Loss of HSP23 causes increased sensitivity to chemical stressors and renders L. donovani non-viable at 37°C. Consequently, HSP23-null mutants are non-infectious to primary macrophages in vitro. All phenotypic effects could be abrogated by the introduction of a functional HSP23 transgene into the null mutant, confirming the specificity of the mutant phenotype. Thus, HSP23 expression is a prerequisite for L. donovani survival at mammalian host temperatures and a crucial virulence factor.
Antje Hombach; Gabi Ommen; Andrea MacDonald; Joachim Clos
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Publication Detail:
Type:  Journal Article     Date:  2014-09-01
Journal Detail:
Title:  Journal of cell science     Volume:  127     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-12-02     Completed Date:  -     Revised Date:  2014-12-02    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  4762-73     Citation Subset:  IM    
Copyright Information:
© 2014. Published by The Company of Biologists Ltd.
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