Document Detail


A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein.
MedLine Citation:
PMID:  15140589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1-->3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution.
Authors:
Graciela C Calabrese; María F Alberto; Rosario Tubio; Mariela M Marani; Marta E Fernández De Recondo; María Lazzari; Eduardo F Recondo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis research     Volume:  113     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  2004  
Date Detail:
Created Date:  2004-05-13     Completed Date:  2004-12-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Elsevier Ltd.
Affiliation:
Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, Ciudad Autónoma de Buenos Aires, 1113, Argentina. gcalabe@ffyb.uba.ar
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MeSH Terms
Descriptor/Qualifier:
Acetylgalactosamine / chemistry
Animals
Anticoagulants / chemistry*,  isolation & purification,  metabolism,  pharmacology*
Calcium / chemistry
Chemical Precipitation
Complement C1 / chemistry,  isolation & purification,  metabolism*
Dermatan Sulfate / chemistry*,  isolation & purification,  metabolism,  pharmacology*
Fibrinolytic Agents / pharmacology
Hexosamines / chemistry
Iduronic Acid / chemistry
Male
Osmolar Concentration
Rats
Rats, Wistar
Structure-Activity Relationship
Sulfates / chemistry
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Complement C1; 0/Fibrinolytic Agents; 0/Hexosamines; 0/Sulfates; 24967-94-0/Dermatan Sulfate; 31022-50-1/Acetylgalactosamine; 3402-98-0/Iduronic Acid; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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