| A small acidic protein 1 (SMAP1) mediates responses of the Arabidopsis root to the synthetic auxin 2,4-dichlorophenoxyacetic acid. | |
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MedLine Citation:
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PMID: 16923017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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2,4-dichlorophenoxyacetic acid (2,4-D), a chemical analogue of indole-3-acetic acid (IAA), is widely used as a growth regulator and exogenous source of auxin. Because 2,4-D evokes physiological and molecular responses similar to those evoked by IAA, it is believed that they share a common response pathway. Here, we show that a mutant, antiauxin resistant1 (aar1), identified in a screen for resistance to the anti-auxin p-chlorophenoxy-isobutyric acid (PCIB), is resistant to 2,4-D, yet nevertheless responds like the wild-type to IAA and 1-napthaleneacetic acid in root elongation and lateral root induction assays. That the aar1 mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the DR5:GUS and HS:AXR3NT-GUS backgrounds, as well as by real-time PCR quantification of IAA11 expression. The two characterized aar1 alleles both harbor multi-gene deletions; however, 2,4-D responsiveness was restored by transformation with one of the genes missing in both alleles, and the 2,4-D-resistant phenotype was reproduced by decreasing the expression of the same gene in the wild-type using an RNAi construct. The gene encodes a small, acidic protein (SMAP1) with unknown function and present in plants, animals and invertebrates but not in fungi or prokaryotes. Taken together, these results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D, and that responses to 2,4-D and IAA are partially distinct. |
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Authors:
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Abidur Rahman; Akari Nakasone; Tory Chhun; Chiharu Ooura; Kamal Kanti Biswas; Hirofumi Uchimiya; Seiji Tsurumi; Tobias I Baskin; Atsushi Tanaka; Yutaka Oono |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 47 ISSN: 0960-7412 ISO Abbreviation: Plant J. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-22 Completed Date: 2006-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 788-801 Citation Subset: IM |
Affiliation:
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Research Group for Plant Resource Application, Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dichlorophenoxyacetic Acid
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pharmacology* Alleles Amino Acid Sequence Arabidopsis / drug effects, genetics, metabolism* Arabidopsis Proteins / genetics, metabolism, physiology* Chromosome Mapping Gene Expression Regulation, Plant Germination Glucuronidase / analysis Indoleacetic Acids / pharmacology Molecular Sequence Data Mutation Plant Growth Regulators / pharmacology* Plant Roots / drug effects, growth & development, metabolism RNA Interference Recombinant Fusion Proteins / analysis Sequence Alignment Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Indoleacetic Acids; 0/Plant Growth Regulators; 0/Recombinant Fusion Proteins; 0/SMAP1 protein, Arabidopsis; 87-51-4/indoleacetic acid; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC 3.2.1.31/Glucuronidase |
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