Document Detail


The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
MedLine Citation:
PMID:  12060788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical slices. We have used this slice overlay assay to identify some of the extracellular cues that regulate differentiation and patterning of axons and dendrites in the cerebral cortex. This assay can be easily adapted to identify factors that control other aspects of neuronal development, such as proliferation and survival, and can also be used to evaluate the role of extracellular signals in the development of nonneural tissues.
Authors:
Franck Polleux; Anirvan Ghosh
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Publication Detail:
Type:  Journal Article     Date:  2002-06-11
Journal Detail:
Title:  Science's STKE : signal transduction knowledge environment     Volume:  2002     ISSN:  1525-8882     ISO Abbreviation:  Sci. STKE     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-12     Completed Date:  2002-07-12     Revised Date:  2009-10-21    
Medline Journal Info:
Nlm Unique ID:  100964423     Medline TA:  Sci STKE     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pl9     Citation Subset:  IM    
Affiliation:
INSERM U371, 18 avenue Doyen Lépine, 69675 BRON Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics
Animals
Brain / embryology,  metabolism
Brain Chemistry
Carbocyanines / metabolism
Cell Differentiation / physiology
Extracellular Matrix / physiology*
Fluorescent Antibody Technique / methods
Fluorescent Dyes / metabolism
Green Fluorescent Proteins
Luminescent Proteins / biosynthesis,  genetics
Mice
Mice, Transgenic
Neurons / chemistry,  cytology*,  physiology*
Promoter Regions, Genetic / genetics
Rats
Recombinant Fusion Proteins / biosynthesis,  genetics
Signal Transduction / physiology*
Chemical
Reg. No./Substance:
0/Actins; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 0/didodecylindocarbocyanine; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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