| The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development. | |
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MedLine Citation:
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PMID: 12060788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical slices. We have used this slice overlay assay to identify some of the extracellular cues that regulate differentiation and patterning of axons and dendrites in the cerebral cortex. This assay can be easily adapted to identify factors that control other aspects of neuronal development, such as proliferation and survival, and can also be used to evaluate the role of extracellular signals in the development of nonneural tissues. |
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Authors:
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Franck Polleux; Anirvan Ghosh |
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Publication Detail:
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Type: Journal Article Date: 2002-06-11 |
Journal Detail:
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Title: Science's STKE : signal transduction knowledge environment Volume: 2002 ISSN: 1525-8882 ISO Abbreviation: Sci. STKE Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-12 Completed Date: 2002-07-12 Revised Date: 2009-10-21 |
Medline Journal Info:
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Nlm Unique ID: 100964423 Medline TA: Sci STKE Country: United States |
Other Details:
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Languages: eng Pagination: pl9 Citation Subset: IM |
Affiliation:
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INSERM U371, 18 avenue Doyen Lépine, 69675 BRON Cedex, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics Animals Brain / embryology, metabolism Brain Chemistry Carbocyanines / metabolism Cell Differentiation / physiology Extracellular Matrix / physiology* Fluorescent Antibody Technique / methods Fluorescent Dyes / metabolism Green Fluorescent Proteins Luminescent Proteins / biosynthesis, genetics Mice Mice, Transgenic Neurons / chemistry, cytology*, physiology* Promoter Regions, Genetic / genetics Rats Recombinant Fusion Proteins / biosynthesis, genetics Signal Transduction / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 0/didodecylindocarbocyanine; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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