Document Detail


The size of the nucleus increases as yeast cells grow.
MedLine Citation:
PMID:  17596521     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is not known how the volume of the cell nucleus is set, nor how the ratio of nuclear volume to cell volume (N/C) is determined. Here, we have measured the size of the nucleus in growing cells of the budding yeast Saccharomyces cerevisiae. Analysis of mutant yeast strains spanning a range of cell sizes revealed that the ratio of average nuclear volume to average cell volume was quite consistent, with nuclear volume being approximately 7% that of cell volume. At the single cell level, nuclear and cell size were strongly correlated in growing wild-type cells, as determined by three different microscopic approaches. Even in G1-phase, nuclear volume grew, although it did not grow quite as fast as overall cell volume. DNA content did not appear to have any immediate, direct influence on nuclear size, in that nuclear size did not increase sharply during S-phase. The maintenance of nuclear size did not require continuous growth or ribosome biogenesis, as starvation and rapamycin treatment had little immediate impact on nuclear size. Blocking the nuclear export of new ribosomal subunits, among other proteins and RNAs, with leptomycin B also had no obvious effect on nuclear size. Nuclear expansion must now be factored into conceptual and mathematical models of budding yeast growth and division. These results raise questions as to the unknown force(s) that expand the nucleus as yeast cells grow.
Authors:
Paul Jorgensen; Nicholas P Edgington; Brandt L Schneider; Ivan Rupes; Mike Tyers; Bruce Futcher
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-06-27
Journal Detail:
Title:  Molecular biology of the cell     Volume:  18     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-24     Completed Date:  2007-11-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3523-32     Citation Subset:  IM    
Affiliation:
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Cell Nucleolus / metabolism
Cell Nucleus / metabolism*,  ultrastructure
Cell Size
Green Fluorescent Proteins / metabolism
Karyopherins / metabolism
Microscopy, Electron
Mutation / genetics
Nuclear Localization Signals
Organelle Size*
Receptors, Cytoplasmic and Nuclear / metabolism
Ribosomes / metabolism
Saccharomyces cerevisiae / cytology*,  growth & development*,  ultrastructure
Grant Support
ID/Acronym/Agency:
GM039978/GM/NIGMS NIH HHS; GM077874/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Karyopherins; 0/Nuclear Localization Signals; 0/Receptors, Cytoplasmic and Nuclear; 0/exportin 1 protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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