| A single gene (Eu4) encodes the tissue-ubiquitous urease of soybean. | |
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MedLine Citation:
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PMID: 7907165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We sought to determine the genetic basis of expression of the ubiquitous (metabolic) urease of soybean. This isozyme is termed the metabolic urease because its loss, in eu4/eu4 mutants, leads to accumulation of urea, whereas loss of the embryo-specific urease isozyme does not. The eu4 lesion eliminated the expression of the ubiquitous urease in vegetative and embryonic tissues. RFLP analysis placed urease clone LC4 near, or within, the Eu4 locus. Sequence comparison of urease proteins (ubiquitous and embryo-specific) and clones (LC4 and LS1) indicated that LC4 and LS1 encode ubiquitous and embryo-specific ureases, respectively. That LC4 is transcribed into poly(A)+ RNA in all tissues was indicated by the amplification of its transcript by an LC4-specific PCR primer. (The LS1-specific primer, on the other hand, amplified poly(A)+ RNA only from developing embryos expressing the embryo-specific urease.) These observations are consistent with Eu4 being the ubiquitous urease structural gene contained in the LC4 clone. In agreement with this notion, the mutant phenotype of eu4/eu4 callus was partially corrected by the LC4 urease gene introduced by particle bombardment. |
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Authors:
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R S Torisky; J D Griffin; R L Yenofsky; J C Polacco |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 242 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-04-06 Completed Date: 1994-04-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY |
Other Details:
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Languages: eng Pagination: 404-14 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Missouri-Columbia, Columbia 65211. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/S69179 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Genes, Plant* Isoenzymes Molecular Sequence Data Mutation Poly A / metabolism Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Seeds / enzymology Sequence Alignment Soybeans / genetics* Urease / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 24937-83-5/Poly A; EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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