Document Detail


A single exposure to hyperbaric oxygen increases levels of circulating nucleosomes but does not induce mononuclear cell apoptosis in divers.
MedLine Citation:
PMID:  19462751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent reports that hyperbaric oxygenation (HBO2) induced apoptosis in T-cell lines raised concern about a possible immunosuppressive effect of HBO2. Nucleosomes, DNA fragments wrapped around a histone core, have been observed in the circulation in diseases with increased cell death such as sepsis. Our aim was to investigate, whether HBO2 increases circulating nucleosomes as a marker of cell death and induces apoptosis of peripheral blood mononuclear cells in vivo. After informed consent 29 healthy volunteers were exposed to a 30 minute dive at 2.8 atmospheres absolute in a pressure chamber under resting conditions, while breathing 100% oxygen. Samples were obtained before and 24 hours after exposure. Circulating nucleosomes were measured in serum. Caspase-3 activation, Bcl-2 expression and mRNA of Bcl-2, Bcl-xl and Bax were analyzed in mononuclear cell extracts. Nucleosomes were elevated markedly 24h after exposure (p<0.01), while caspase-3 was not activated significantly. mRNA levels of Bcl-2, Bcl-xl and Bax were not altered. In conclusion, while evidence of elevated levels of circulating nucleosomes was found, mononuclear cell apoptosis was not affected by a single exposure to hyperbaric oxygen.
Authors:
S U Weber; A Koch; U Siekmann; C Neitzel; F Stüber; A Hoeft; S Schröder
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc     Volume:  36     ISSN:  1066-2936     ISO Abbreviation:  Undersea Hyperb Med     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-05-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312954     Medline TA:  Undersea Hyperb Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  117-25     Citation Subset:  IM; S    
Affiliation:
Department of Anesthesiology and Intensive Care Medicine, University of Bonn Medical Center, Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Apoptosis / immunology,  physiology*
Caspase 3 / metabolism
Enzyme Activation
Humans
Hyperbaric Oxygenation / adverse effects*
Leukocytes, Mononuclear / physiology*
Male
Nucleosomes / metabolism*
Polymerase Chain Reaction / methods
Prospective Studies
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
RNA, Messenger / metabolism
Time Factors
Young Adult
bcl-2-Associated X Protein / metabolism
bcl-X Protein / metabolism
Chemical
Reg. No./Substance:
0/Nucleosomes; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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