Document Detail


A single-component multidrug transporter of the major facilitator superfamily is part of a network that protects Escherichia coli from bile salt stress.
MedLine Citation:
PMID:  24684269     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Resistance to high concentrations of bile salts in the human intestinal tract is vital for the survival of enteric bacteria such as Escherichia coli. Although the tripartite AcrAB-TolC efflux system plays a significant role in this resistance, it is purported that other efflux pumps must also be involved. We provide evidence from a comprehensive suite of experiments performed at two different pH values (7.2 and 6.0) that reflect pH conditions that E. coli may encounter in human gut that MdtM, a single-component multidrug resistance transporter of the major facilitator superfamily, functions in bile salt resistance in E. coli by catalysing secondary active transport of bile salts out of the cell cytoplasm. Furthermore, assays performed on a chromosomal ΔacrB mutant transformed with multicopy plasmid encoding MdtM suggested a functional synergism between the single-component MdtM transporter and the tripartite AcrAB-TolC system that results in a multiplicative effect on resistance. Substrate binding experiments performed on purified MdtM demonstrated that the transporter binds to cholate and deoxycholate with micromolar affinity, and transport assays performed on inverted vesicles confirmed the capacity of MdtM to catalyse electrogenic bile salt/H(+) antiport.
Authors:
Stephanie Paul; Kamela O Alegre; Scarlett R Holdsworth; Matthew Rice; James A Brown; Paul McVeigh; Sharon M Kelly; Christopher J Law
Related Documents :
20565959 - Array comparative hybridisation reveals a high degree of similarity between uk and euro...
19899619 - Genetic features of clinical pseudomonas aeruginosa strains.
7542269 - Detection of the turkey pathogens mycoplasma meleagridis and m. iowae by amplification ...
16052279 - Rapid detection and differentiation of newcastle disease virus by real-time pcr with me...
10488759 - Molecular diagnosis of group b coltiviruses infections.
12324369 - Detection of helicobacter colonization of the murine lower bowel by genus-specific pcr-...
11292769 - Periodontal pathogens produce quorum sensing signal molecules.
17483259 - Isolation of key methanogens for global methane emission from rice paddy fields: a nove...
12764569 - Functional analyses of genes involved in the metabolism of ferulic acid in pseudomonas ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-31
Journal Detail:
Title:  Molecular microbiology     Volume:  -     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-4-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The evolution of seed dormancy: environmental cues, evolutionary hubs, and diversification of the se...
Next Document:  Basic quinolinonyl diketo acid derivatives as inhibitors of HIV integrase and their activity against...