Document Detail


A single-cell-based model of tumor growth in vitro: monolayers and spheroids.
MedLine Citation:
PMID:  16224119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To what extent the growth dynamics of tumors is controlled by nutrients, biomechanical forces and other factors at different stages and in different environments is still largely unknown. Here we present a biophysical model to study the spatio-temporal growth dynamics of two-dimensional tumor monolayers and three-dimensional tumor spheroids as a complementary tool to in vitro experiments. Within our model each cell is represented as an individual object and parametrized by cell-biophysical and cell-kinetic parameters that can all be experimentally determined. Hence our modeling strategy allows us to study which mechanisms on the microscopic level of individual cells may affect the macroscopic properties of a growing tumor. We find the qualitative growth kinetics and patterns at early growth stages to be remarkably robust. Quantitative comparisons between computer simulations using our model and published experimental observations on monolayer cultures suggest a biomechanically-mediated form of growth inhibition during the experimentally observed transition from exponential to sub-exponential growth at sufficiently large tumor sizes. Our simulations show that the same transition during the growth of avascular tumor spheroids can be explained largely by the same mechanism. Glucose (or oxygen) depletion seems to determine mainly the size of the necrotic core but not the size of the tumor. We explore the consequences of the suggested biomechanical form of contact inhibition, in order to permit an experimental test of our model. Based on our findings we propose a phenomenological growth law in early expansion phases in which specific biological small-scale processes are subsumed in a small number of effective parameters.
Authors:
Dirk Drasdo; Stefan Höhme
Related Documents :
19345189 - A mutant-p53/smad complex opposes p63 to empower tgfbeta-induced metastasis.
20145179 - Anti-transforming growth factor beta receptor ii antibody has therapeutic efficacy agai...
21041659 - Autocrine tgf-beta and stromal cell-derived factor-1 (sdf-1) signaling drives the evolu...
15795099 - Activation of the coagulation system in cancerogenesis and metastasation.
19064739 - Stem cell concepts renew cancer research.
16197719 - Biology of osteogenic sarcoma.
17631279 - Mechanism of acridine-based telomerase inhibition and telomere shortening.
23618379 - Wt1 microdeletion and slowly progressing focal glomerulosclerosis in a patient with mal...
22466849 - Chemistry and biological activities of terpenoids from copaiba (copaifera spp.) oleores...
Publication Detail:
Type:  Journal Article     Date:  2005-07-12
Journal Detail:
Title:  Physical biology     Volume:  2     ISSN:  1478-3975     ISO Abbreviation:  Phys Biol     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-10-14     Completed Date:  2007-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197454     Medline TA:  Phys Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  133-47     Citation Subset:  IM    
Affiliation:
Interdisciplinary Centre for Bioinformatics, University of Leipzig, Härtelstr. 16-18, D-04107 Leipzig, Germany. drasdo@izbi.uni-leipzig.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biomechanics
Cell Proliferation
Computer Simulation
Contact Inhibition
Food
Humans
Kinetics
Models, Biological*
Neoplasms / pathology*
Spheroids, Cellular / pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  How do biomolecular systems speed up and regulate rates?
Next Document:  Phosphatase localization in bacterial chemotaxis: divergent mechanisms, convergent principles.