Document Detail


A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS.
MedLine Citation:
PMID:  3046615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genes for lanosterol 14-demethylase, cytochrome P450(14DM), and a mutated inactive cytochrome P450SG1 were cloned from S. cerevisiae strains D587 and SG1, respectively. A single nucleotide change resulting in substitution of Asp for Gly-310 of cytochrome P450(14DM) was found to have occurred in cytochrome P450SG1. In this protein the 6th ligand to heme iron is a histidine residue instead of a water molecule, which may be the ligand for the active cytochrome P450(14DM). Molecular models of the active sites of the cytochrome P450(14DM) and cytochrome P450SG1 were built by computer modeling on the basis of the known structure of that of cytochrome P450CAM whose crystallographic data are available. The mechanisms which may cause a histidine residue to gain access to the heme iron are discussed.
Authors:
N Ishida; Y Aoyama; R Hatanaka; Y Oyama; S Imajo; M Ishiguro; T Oshima; H Nakazato; T Noguchi; U S Maitra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  155     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-10-05     Completed Date:  1988-10-05     Revised Date:  2010-04-06    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  317-23     Citation Subset:  IM    
Affiliation:
Suntory Institute for Biomedical Research, Osaka, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M21483;  M21484
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cloning, Molecular*
Cytochrome P-450 Enzyme System / genetics*,  isolation & purification
DNA / isolation & purification*
Fungal Proteins / genetics,  isolation & purification
Genes
Genes, Fungal*
Humans
Infant, Newborn
Molecular Sequence Data
Mutation*
Oxidoreductases / genetics
Saccharomyces cerevisiae / genetics
Chemical
Reg. No./Substance:
0/Fungal Proteins; 9007-49-2/DNA; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 1.14.13.70/sterol 14-demethylase

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