Document Detail


A single amino acid in human APOBEC3F alters susceptibility to HIV-1 Vif.
MedLine Citation:
PMID:  20971849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human APOBEC3F (huA3F) potently restricts the infectivity of HIV-1 in the absence of the viral accessory protein virion infectivity factor (Vif). Vif functions to preserve viral infectivity by triggering the degradation of huA3F but not rhesus macaque A3F (rhA3F). Here, we use a combination of deletions, chimeras, and systematic mutagenesis between huA3F and rhA3F to identify Glu(324) as a critical determinant of huA3F susceptibility to HIV-1 Vif-mediated degradation. A structural model of the C-terminal deaminase domain of huA3F indicates that Glu(324) is a surface residue within the α4 helix adjacent to residues corresponding to other known Vif susceptibility determinants in APOBEC3G and APOBEC3H. This structural clustering suggests that Vif may bind a conserved surface present in multiple APOBEC3 proteins.
Authors:
John S Albin; Rebecca S LaRue; Jessalyn A Weaver; William L Brown; Keisuke Shindo; Elena Harjes; Hiroshi Matsuo; Reuben S Harris
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-22
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-20     Completed Date:  2011-01-24     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  40785-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Substitution
Animals
Cell Line
Cytosine Deaminase / genetics,  metabolism*
Genetic Predisposition to Disease / genetics
HIV Infections / genetics,  metabolism*
HIV-1 / genetics,  metabolism*
Humans
Macaca mulatta
Protein Binding / genetics
Protein Structure, Secondary
Protein Structure, Tertiary
Simian immunodeficiency virus / genetics,  metabolism
vif Gene Products, Human Immunodeficiency Virus / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
F30 DA026310/DA/NIDA NIH HHS; F30 DA026310/DA/NIDA NIH HHS; R01 AI064046/AI/NIAID NIH HHS; R33 AI073167/AI/NIAID NIH HHS; T32 GM008244/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/vif Gene Products, Human Immunodeficiency Virus; 0/vif protein, Human immunodeficiency virus 1; EC 3.5.4.1/APOBEC3 protein, human; EC 3.5.4.1/Cytosine Deaminase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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