Document Detail

The simultaneous measurement of low rates of CO2 and O2 exchange in biological systems.
MedLine Citation:
PMID:  9417789     Owner:  NLM     Status:  MEDLINE    
An instrument for measuring low rates of biological O2 exchange using an open-flow gas analysis system is described. A novel differential O2 sensor that is capable of measuring as little as 0.4 Pa O2 against a back-ground of ambient air (20,900 Pa O2), yet has a dynamic range of +/- 2000 Pa O2 (i.e., +/- ca. 2% O2) is described. Baseline drift was typically less than 0.025 Pa min-1. The differential O2 sensor was incorporated into a respiratory quotient/photosynthetic quotient analyzer that contained other environmental sensors for atmospheric pressure, absolute O2 and CO2 concentration, temperature of the differential O2 sensor block, and differential pressure between reference and sample streams. Protocols for how these sensors can be used to calibrate the differential O2 sensor and to improve its stability with time are described. Together, the differential O2 sensor, the environmental sensors, and the simple calibration techniques allow for simultaneous, noninvasive, and accurate measurements of O2 and CO2 exchange in tissues with metabolic rates as low as about 0.1 mumol O2 or CO2 h-1. Example data are provided in which O2 differentials of 3 to 41 Pa O2 were measured in an open-flow system.
J R Willms; A N Dowling; Z M Dong; S Hunt; B J Shelp; D B Layzell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  254     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-06     Completed Date:  1998-02-06     Revised Date:  2006-04-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  272-82     Citation Subset:  IM    
Department of Biology, Queen's University, Kingston, Ontario, Canada.
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MeSH Terms
Carbon Dioxide / metabolism*
Computational Biology / instrumentation,  methods*
Insects / metabolism
Models, Biological
Oxygen / metabolism*
Photosynthesis / physiology
Plants / metabolism
Reference Values
Reg. No./Substance:
0/Soil; 124-38-9/Carbon Dioxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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