Document Detail

The simultaneous analysis of proteins, lipids, and diterpenoid resins found in cultural objects.
MedLine Citation:
PMID:  17867529     Owner:  NLM     Status:  MEDLINE    
We report a GC-MS method for the simultaneous analysis of proteins oil, and diterpenoid resins found in cultural objects. The method was initially designed for protein analysis of protenaceous paints and adhesives and involves acid hydrolysis as the first step. The amino acids in the protein hydrolysates, thus obtained, are treated with propan-l-ol/ hydrogen chloride and then pentafluoropropionic anhydride. The procedure was found also to yield the propyl esters of fatty acids derived from lipids and diterpenoid acids derived from natural resins, and thus allows the choice of a single method for the analysis of artists media which contain either oil s or proteins or mixtures of both proteins and oils or even resins. Thus natural mixtures such as egg yolk and also mixtures made by the artist such as animal glue/seed oil emulsions can be analysed. Coupled with FTIR analysis of paints and the staining of cross sections, to indicate layer structure the method can help to elucidate the paints and adhesives used by artists.
Brian Singer; Ross McGuigan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annali di chimica     Volume:  97     ISSN:  0003-4592     ISO Abbreviation:  Ann Chim     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-10-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7610375     Medline TA:  Ann Chim     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  405-17     Citation Subset:  IM    
School of Arts and Social Sciences/School of Applied Science, Northumbria University, Newcastle upon Tyne, England, UK.
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MeSH Terms
Diterpenes / analysis*
Lipids / analysis*
Proteins / analysis*
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared
Reg. No./Substance:
0/Diterpenes; 0/Lipids; 0/Proteins

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