Document Detail


A simplified protocol for fluorescence in situ hybridization with repetitive DNA probes and its use in clinical cytogenetics.
MedLine Citation:
PMID:  1884517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for chromosome-specific staining and its use in clinical cytogenetics is described. This fluorescence in situ hybridization protocol for repetitive DNA probes results in yellow-green fluorescent signals on orange-red stained chromosomes. Special characteristics are its simplicity, the use of digoxigenin-11-dUTP for labeling, and the combination of high stringency criteria for hybridization and low stringency for washing. The method is particularly advantageous in cases with structurally abnormal extra chromosomes (ESACs), marker chromosomes of gonosomal origin, and chromosomal mosaicism. It may also facilitate the screening of cases for fragile X. The chromosome-specific staining can be done within 1 working-day.
Authors:
O Bartsch; E Schwinger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical genetics     Volume:  40     ISSN:  0009-9163     ISO Abbreviation:  Clin. Genet.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0253664     Medline TA:  Clin Genet     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  47-56     Citation Subset:  IM    
Affiliation:
Department of Human Genetics, Medizinische Universität zu Lübeck, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Aberrations* / diagnosis*
Chromosome Disorders*
Chromosomes / ultrastructure*
Clinical Protocols / standards*
DNA Probes / diagnostic use*
Deoxyuracil Nucleotides
Digoxigenin / diagnostic use*
Female
Fluorescent Dyes / diagnostic use*
Humans
Male
Mosaicism
Nucleic Acid Hybridization*
Repetitive Sequences, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA Probes; 0/Deoxyuracil Nucleotides; 0/Fluorescent Dyes; 1672-46-4/Digoxigenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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