Document Detail


A simplified cobalt-lysine method for tracing axon trajectories in the central nervous system of vertebrates.
MedLine Citation:
PMID:  6521959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cobalt-lysine method for tract tracing in the vertebrate CNS has been shortened and simplified to the following steps: (1) the cobalt-lysine is applied to the nerve or tract being studied; (2) the animal is perfused 1-2 days later by the following sequence of solutions: (a) phosphate-buffered ammonium sulfide, (b) phosphate buffer alone and (c) phosphate-buffered glutaraldehyde; (3) the brain is exposed to the fixative overnight and left in 30% sugar another night; (4) the brain is then frozen and cut on a cryostat; the sections being collected directly on slides; (5) the mounted sections are then put in an intensification solution before dehydration, clearing and coverslipping.
Authors:
G T Bazer; S O Ebbesson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  51     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-03-21     Completed Date:  1985-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  315-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Ambystoma
Animals
Central Nervous System / anatomy & histology*
Fishes / anatomy & histology
Neuroanatomy / methods*
Visual Pathways / anatomy & histology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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