| A simple test for the detection of KPC and metallo-β-lactamase carbapenemase-producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. | |
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MedLine Citation:
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PMID: 21689207 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Clin Microbiol Infect ABSTRACT: We evaluated the ability of the combination disk test (CDT) and the Modified Hodge Test (MHT) to discriminate between various carbapenemase-producing Pseudomonas aeruginosa isolates (KPC, n = 36; metallo-β-lactamase (MBL), n = 38) and carbapenemase non-producers (n = 75). For the CDT, the optimal inhibitor concentrations and cut-off values were: 600 μg of 3-aminophenylboronic acid (APB) per disk (an increment of ≥4 mm), 1000 μg of dipicolinic acid (DPA) per disk (an increment of ≥5 mm) and 3000 μg of cloxacillin per disk (an increment of ≥3 mm). APB had excellent sensitivity (97%) and specificity (97%) for the detection of KPC enzymes. DPA detected MBL enzymes with a sensitivity and specificity of 97% and 81%, respectively. The MHT resulted in a low sensitivity (78%) and specificity (57%). The CDT could be very useful in daily practice to provide fast and reliable detection of KPC and MBL carbapenemases among P. aeruginosa isolates. |
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Authors:
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F Pasteran; O Veliz; D Faccone; L Guerriero; M Rapoport; T Mendez; A Corso |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-24 |
Journal Detail:
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Title: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases Volume: - ISSN: 1469-0691 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-6-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9516420 Medline TA: Clin Microbiol Infect Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 European Society of Clinical Microbiology and Infectious Diseases. No claim to original US government works. |
Affiliation:
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Servicio Antimicrobianos, Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas (INEI) - ANLIS 'Dr Carlos G. Malbrán', Ministerio de Salud y Ambiente, Ciudad Autónoma de Buenos Aires, Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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