| A simple technique for collecting blood of testicular origin: application to in vivo studies on testicular steroidogenesis in rats and Macaca fascicularis. | |
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MedLine Citation:
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PMID: 2069857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A technique for rapidly collecting blood of testicular origin is described, one which can provide sufficient plasma amounts to investigate some steps of testicular steroid biogenesis in vivo in 2 species. In adult male rats, testosterone (T), androstenedione (4A) and 5-androstenediol (5AD) were determined in pampiniform plexus testicular venous blood (PPTV) and peripheral (PV) blood samples before and 2 h after human Chorionic Gonadotropin (hCG). PPTV concentration of 5AD was 0.83 +/- 0.1 ng/ml (mean +/- SEM) with a PPTV/PV ratio of 7.0 +/- 1.0, comparable to a PPTV/PV ratio for 4A of 5.8 +/- 1.8. After hCG, PPTV concentration of 5AD significantly increased to 1.28 +/- 0.15 ng/ml (P less than 0.05). Those data are in favor of a participation of 5-ene pathway to testicular biogenesis of T associated to a 4-ene pathway which is predominant. In adult male Macaca fascicularis, spermatic vein (SV) concentrations of 5AD and 4A were comparable (3.0 +/- 1.2 vs 4.3 +/- 1.0 ng/ml) as well as SV/PV ratios under basal conditions (3.5 +/- 0.9 vs 5.1 +/- 0.1), as well as 48 h after hCG, confirming in vivo that both 5-ene and 4-ene pathways are involved in testicular T biogenesis. Testicular production of estradiol (E2), estrone (E1) and their sulfates E2S and E1S showed a SV/PV ratio significantly higher than 1 (3.4 +/- 0.6; 2.4 +/- 0.1; 1.7 +/- 0.2 and 1.6 +/- 0.2, respectively). |
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Authors:
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C Blacker; A Akar; R Scholler |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of steroid biochemistry and molecular biology Volume: 39 ISSN: 0960-0760 ISO Abbreviation: J. Steroid Biochem. Mol. Biol. Publication Date: 1991 Jul |
Date Detail:
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Created Date: 1991-08-20 Completed Date: 1991-08-20 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9015483 Medline TA: J Steroid Biochem Mol Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 105-13 Citation Subset: IM |
Affiliation:
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Fondation de Recherche en Hormonologie, Fresnes, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Androstenediol
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blood Androstenedione / blood Androstenols / blood* Animals Blood Specimen Collection / methods* Macaca fascicularis Male Rats Rats, Inbred Strains Testis / drug effects, metabolism* Testosterone / blood |
| Chemical | |
Reg. No./Substance:
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0/Androstenols; 521-17-5/Androstenediol; 58-22-0/Testosterone; 63-05-8/Androstenedione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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