Document Detail


A simple technique for the analysis of positional distribution of fatty acids on di- and triacylglycerols using lipase and phospholipase A2.
MedLine Citation:
PMID:  7666017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple technique is described for the analysis of positional distribution of fatty acids on di- and triacylglycerols using lipase and phospholipase A2 that de-esterify fatty acids from specific sn positions. The technique makes use of the fact that methanolic-NaOH methylates only fatty acids esterified to glycerol, while methanolic-HCl methylates both free and esterified fatty acids. After lipase action it is possible to determine the fatty acid released by lipase activity by comparing the fatty acid contents of the two methylation reactions. A computer program has been written to calculate enzyme activity and positional distribution from the results. The new technique is easier to use as it eliminates thin-layer chromatography used in the standard technique and can be performed on smaller samples using less lipase.
Authors:
J P Williams; M U Khan; D Wong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  36     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-10     Completed Date:  1995-10-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1407-12     Citation Subset:  IM    
Affiliation:
Department of Botany, University of Toronto, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Brassica / chemistry
Diglycerides / analysis*,  chemistry
Esterification
Fatty Acids / analysis*,  metabolism
Glycerol / metabolism
Lipase / metabolism*
Methylation
Phosphatidylcholines / analysis
Phosphatidylethanolamines / analysis
Phosphatidylglycerols / analysis
Phospholipases A / metabolism*
Phospholipases A2
Software
Triglycerides / analysis*,  chemistry
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phosphatidylglycerols; 0/Triglycerides; 56-81-5/Glycerol; EC 3.1.1.-/Phospholipases A; EC 3.1.1.3/Lipase; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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