Document Detail

A simple survival model of volume-controlled hemorrhagic shock in awake rats.
MedLine Citation:
PMID:  1650026     Owner:  NLM     Status:  MEDLINE    
A simple rat model was developed for the study of spontaneous survival after volume-controlled hemorrhage. The objective was to determine in awake, unrestrained rats the shed blood volume (SBV) in ml/100 g body weight that without fluid resuscitation, would result in either a high or a low percentage of survivors within 24 h. About 24 h after cannulation under light anesthesia, the awake rats were insulted with arterial blood withdrawal at a constant rate over 20 min, while mean arterial pressure (MAP) was monitored (N = 78). Then, the arterial catheter was removed, and the rats were observed for 24 h or until death. With increasing SBV, survival rate decreased, SBV of 2.50 ml/100 g (group I) resulted in 74% survivors at 2 h and 24 h; SBV of 2.75 ml/100 g (group II), in 67% survivors at 2 h and 46% at 24 h; SBV of 3.00 ml/100 g (group III), in 35% survivors at 2 h and 20% at 24 h; and SBV of 3.50 ml/100 g (group IV), in no survivors to 2 h. MAP declined similarly over 20 min blood withdrawal in the four insult groups, without difference between ultimate survivors vs. nonsurvivors. All rats survived to the end of 20 min hemorrhage (i.e. hemorrhagic shock [HS] time = 0 min). Deaths at HS time 0-2 h occurred after SBV of 2.50 ml/100 g, at HS time 56 +/- 35 min; after SBV of 2.75 ml/100 g, at HS 81 +/- 26 min; after SBV of 3.00 ml/100 g, at HS 37 +/- 33 min; and after SBV of 3.50 ml/100 g, at HS 11 +/- 2 min. Weight may have affected MAP response and survival. We conclude that a volume-controlled HS model in rats without anesthesia or restraint is feasible. SBV of 2.50 ml/100 g should be suitable for testing additional insults. SBV of 3.00 ml/100 g should be suitable for testing resuscitative therapies. The model should be modified to allow monitoring of key variables after hemorrhage.
M J McGlew; P Safar; P Stremple
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Resuscitation     Volume:  21     ISSN:  0300-9572     ISO Abbreviation:  Resuscitation     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-08-23     Completed Date:  1991-08-23     Revised Date:  2009-08-25    
Medline Journal Info:
Nlm Unique ID:  0332173     Medline TA:  Resuscitation     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  247-57     Citation Subset:  IM    
Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Pittsburgh, PA 15260.
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MeSH Terms
Blood Pressure / physiology
Blood Volume
Rats, Inbred Strains
Resuscitation / methods
Shock, Hemorrhagic / mortality*
Survival Rate
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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