Document Detail


A simple and sensitive fluorometric assay method for taurine using high-voltage paper electrophoresis.
MedLine Citation:
PMID:  1021602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and sensitive fluorometric assay method for taurine (2-aminoethanesulfonic acid) has been developed. For the separation of taurine, high voltage paper electrophoresis subsequent to column chromatographic procedures was employed. Fluorescent product of taurine was yielded by spraying fluorescamine (4-phenylspiro [furan-2(3H), 1'-phthalan]-3, 3'-dione) and borate buffer on the paper, and the fluorescence was assayed spectro-fluorometrically after eluting with 50% ethanol. The linear relationship between the concentration of taurine and fluorescence developed was achieved over the concentration ranges of 0.5-10 nmoles, and the recoveries obtained were 90-100%. The specificity of this method for taurine was satisfactory and structural analogues involved in the metabolic pathway of taurine did not interfere with the assay. Examples for tissue levels of taurine in various organs of the rat as determined by this new method are also presented.
Authors:
K Yoshikawa; K Kuriyama
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of pharmacology     Volume:  26     ISSN:  0021-5198     ISO Abbreviation:  Jpn. J. Pharmacol.     Publication Date:  1976 Dec 
Date Detail:
Created Date:  1977-05-12     Completed Date:  1977-05-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2983305R     Medline TA:  Jpn J Pharmacol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  649-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography
Electrophoresis, Paper / methods
Fluorescamine
Fluorometry
Male
Methods
Rats
Taurine / analysis*
Chemical
Reg. No./Substance:
107-35-7/Taurine; 38183-12-9/Fluorescamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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