| A simple route to single-nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida). | |
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MedLine Citation:
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PMID: 21429159 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Although single-nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida, in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re-sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next-generation sequencing facilities. |
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Authors:
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Morten Tange Olsen; Veronica Hirsh Volny; Martine Bérubé; Rune Dietz; Christian Lydersen; Kit M Kovacs; Richard S Dodd; Per J Palsbøll |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-10 |
Journal Detail:
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Title: Molecular ecology resources Volume: 11 Suppl 1 ISSN: 1755-0998 ISO Abbreviation: Mol Ecol Resour Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465604 Medline TA: Mol Ecol Resour Country: England |
Other Details:
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Languages: eng Pagination: 9-19 Citation Subset: IM |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Evolutionary Genetics Group, Department of Genetics, Microbiology, and Toxicology, Stockholm University, Sweden. mtolsen@gmt.su.se |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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