Document Detail


A simple, reproducible and sensitive spectrophotometric method to estimate microalgal lipids.
MedLine Citation:
PMID:  22483211     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Quantification of total lipids is a necessity for any study of lipid production by microalgae, especially given the current interest in microalgal carbon capture and biofuels. In this study, we employed a simple yet sensitive method to indirectly measure the lipids in microalgae by measuring the fatty acids (FA) after saponification. The fatty acids were reacted with triethanolamine-copper salts (TEA-Cu) and the ternary TEA-Cu-FA complex was detected at 260nm using a UV-visible spectrometer without any colour developer. The results showed that this method could be used to analyse low levels of lipids in the range of nano-moles from as little as 1mL of microalgal culture. Furthermore, the structure of the TEA-Cu-FA complex and related reaction process are proposed to better understand this assay. There is no special instrument required and the method is very reproducible. To the best of our knowledge, this is the first report of the use of UV absorbance of copper salts with FA as a method to estimate lipids in algal cultures. It will pave the way for a more convenient assay of lipids in microalgae and can readily be expanded for estimating lipids in other biological systems.
Authors:
Yimin Chen; Seetharaman Vaidyanathan
Publication Detail:
Type:  Journal Article     Date:  2012-03-06
Journal Detail:
Title:  Analytica chimica acta     Volume:  724     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  67-72     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
ChELSI Institute, Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield S1 3JD, UK.
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