Document Detail

A simple, rapid and yet less expensive method to detect chitinase in agarose plates.
MedLine Citation:
PMID:  17367865     Owner:  NLM     Status:  MEDLINE    
Methods have been developed, in the past, to detect the chitinase activity in plate assays using fluorescent brightener Calcofluor White which is simple and rapid, but expensive. We, now, report the use of a less expensive fluorescent brightener Ranipal, which reduces 2500-times the cost of the assay. The use of Ranipal, in place of Calcofluor White, does not compromise on the sensitivity and limits of detection, but required more washes. This forms the first report on the use of Ranipal for detection of chitinases and also the least expensive method for this purpose.
Kondreddy Anil; Kottapalli Seshagirirao; Appa Rao Podile
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Publication Detail:
Type:  Journal Article     Date:  2007-02-03
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  70     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-07     Completed Date:  2007-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  683-4     Citation Subset:  IM    
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India.
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MeSH Terms
Bacterial Proteins / analysis
Chitinase / analysis*
Fluorescent Dyes
Sensitivity and Specificity
Serratia marcescens / enzymology
Spectrometry, Fluorescence / methods
Reg. No./Substance:
0/Bacterial Proteins; 0/Benzenesulfonates; 0/Fluorescent Dyes; 4404-43-7/C.I. Fluorescent Brightening Agent 28; EC

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