Document Detail


A simple, rapid and yet less expensive method to detect chitinase in agarose plates.
MedLine Citation:
PMID:  17367865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methods have been developed, in the past, to detect the chitinase activity in plate assays using fluorescent brightener Calcofluor White which is simple and rapid, but expensive. We, now, report the use of a less expensive fluorescent brightener Ranipal, which reduces 2500-times the cost of the assay. The use of Ranipal, in place of Calcofluor White, does not compromise on the sensitivity and limits of detection, but required more washes. This forms the first report on the use of Ranipal for detection of chitinases and also the least expensive method for this purpose.
Authors:
Kondreddy Anil; Kottapalli Seshagirirao; Appa Rao Podile
Related Documents :
19909965 - A calculation method for the prediction of effective plate height in capillary gas chro...
7007415 - Quantitative method for enumeration of enterotoxigenic escherichia coli.
7463335 - Impurities in drugs v: meprobamate.
1937505 - Ultrasonic interferometry: study of particle sedimentation in liquid.
21619725 - Pharmacokinetic properties of succinylmonocholine in surgical patients.
20333615 - Direct ms measurement of the extract of ligularia virgaurea collected in yunnan and sic...
Publication Detail:
Type:  Journal Article     Date:  2007-02-03
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  70     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-07     Completed Date:  2007-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  683-4     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis
Benzenesulfonates
Chitinase / analysis*
Fluorescent Dyes
Sensitivity and Specificity
Serratia marcescens / enzymology
Spectrometry, Fluorescence / methods
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Benzenesulfonates; 0/Fluorescent Dyes; 4404-43-7/C.I. Fluorescent Brightening Agent 28; EC 3.2.1.14/Chitinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Point mutations at the local anesthetic receptor site modulate the state-dependent block of rat Na v...
Next Document:  A fast and accurate procedure for deriving the Sholl profile in quantitative studies of neuronal mor...