Document Detail

A simple, rapid and sensitive presence/absence detection test for bacteriophage in drinking water.
MedLine Citation:
PMID:  8486556     Owner:  NLM     Status:  MEDLINE    
A rapid, simple and sensitive direct bacteriophage presence detection method for 500 ml drinking water samples has been developed. The method includes a glass device consisting of a jar containing the water sample and an immersible probe filled with solidified soft agar containing bacterial host cells. Host bacteria in logarithmic phase were added to the experimental volume and the probe was submerged. The entire device was incubated in a water bath at 36 degrees C. Plaques of somatic bacteriophage infecting Escherichia coli strain CN13, could be detected within 3 h. Male-specific bacteriophages infecting E. coli F+ amp were detected within 6 h. Bacteriophage infecting the anaerobe Bacteroides fragilis subsp. fragilis HSP40 were detected after 8 h. Application of this device and the associated technique, enabled a one-step detection of 1 pfu of E. coli or Bact. fragilis specific bacteriophage in 500 ml drinking water samples.
R Armon; Y Kott
Related Documents :
16217926 - Microbiological quality of carbonated drinking water produced with in-home carbonation ...
22367076 - Suppression of twins in b-oriented mfi molecular sieve films under microwave irradiation.
19806756 - A new method for water desalination using microbial desalination cells.
15776216 - Bacterial community shift along a subsurface geothermal water stream in a japanese gold...
24833106 - Bis(2,2,2-trifluoroethyl) ether as an electrolyte co-solvent for mitigating self-discha...
11285916 - Phosphorus mobilization from various sediment pools in response to increased ph and sil...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  74     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-10     Completed Date:  1993-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  490-6     Citation Subset:  IM    
Environmental and Water Resources Engineering, Technion, Haifa, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteriophages / growth & development,  isolation & purification*
Bacteroides fragilis
Coliphages / growth & development,  isolation & purification
Escherichia coli
Evaluation Studies as Topic
Plaque Assay
Sensitivity and Specificity
Software Design
Virology / methods*,  statistics & numerical data
Water Microbiology*
Water Supply
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Bactericidal activity of disinfectants on Listeria.
Next Document:  External quality assessment in water microbiology: statistical analysis of performance.