Document Detail


A simple, rapid and sensitive presence/absence detection test for bacteriophage in drinking water.
MedLine Citation:
PMID:  8486556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid, simple and sensitive direct bacteriophage presence detection method for 500 ml drinking water samples has been developed. The method includes a glass device consisting of a jar containing the water sample and an immersible probe filled with solidified soft agar containing bacterial host cells. Host bacteria in logarithmic phase were added to the experimental volume and the probe was submerged. The entire device was incubated in a water bath at 36 degrees C. Plaques of somatic bacteriophage infecting Escherichia coli strain CN13, could be detected within 3 h. Male-specific bacteriophages infecting E. coli F+ amp were detected within 6 h. Bacteriophage infecting the anaerobe Bacteroides fragilis subsp. fragilis HSP40 were detected after 8 h. Application of this device and the associated technique, enabled a one-step detection of 1 pfu of E. coli or Bact. fragilis specific bacteriophage in 500 ml drinking water samples.
Authors:
R Armon; Y Kott
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  74     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-10     Completed Date:  1993-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  490-6     Citation Subset:  IM    
Affiliation:
Environmental and Water Resources Engineering, Technion, Haifa, Israel.
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MeSH Terms
Descriptor/Qualifier:
Agar
Bacteriophages / growth & development,  isolation & purification*
Bacteroides fragilis
Coliphages / growth & development,  isolation & purification
Escherichia coli
Evaluation Studies as Topic
Plaque Assay
Sensitivity and Specificity
Software Design
Virology / methods*,  statistics & numerical data
Water Microbiology*
Water Supply
Chemical
Reg. No./Substance:
9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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