Document Detail


A simple and rapid method to assay triacylglycerol in cells and tissues.
MedLine Citation:
PMID:  17717377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.
Authors:
Danielle M Schwartz; Nathan E Wolins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-23
Journal Detail:
Title:  Journal of lipid research     Volume:  48     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-15     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2514-20     Citation Subset:  IM    
Affiliation:
Division of Nutritional Science, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Colorimetry / methods
Hela Cells
Humans
Lipoprotein Lipase / metabolism
Microchemistry / methods*
Reproducibility of Results
Triglycerides / analysis*
Chemical
Reg. No./Substance:
0/Triglycerides; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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