Document Detail


A simple and rapid method for removal of specific cell populations from whole blood.
MedLine Citation:
PMID:  8946015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is presented in which specific cells can be rapidly separated from whole blood by the use of antibody labelled dense particles utilizing gravity as a means of separation. This method specifically removes the targeted cells with minimal non-specific cell loss. The method is simple to perform and rapid, requiring less than 10 min for cell/particle binding and separation. The method is robust so that exact ratios of particles to cells are not needed. This method could be used in any separation scheme to remove specific cell populations without significant modification.
Authors:
R K Zwerner; R J Schmittling; T R Russell
Related Documents :
3744735 - Cell body counts in human ciliary ganglia.
16882215 - Laser microdissection separation of pure spermatozoa from epithelial cells for short ta...
25202395 - Comparison of ultracentrifugation and density gradient separation methods for isolating...
25244405 - Ternary blend hybrid solar cells incorporating wide and narrow bandgap polymers.
11559705 - Involvement of the interaction between p21 and proliferating cell nuclear antigen for t...
23730415 - Mri detection of nonproliferative tumor cells in lymph node metastases using iron oxide...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  198     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-01-07     Completed Date:  1997-01-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-202     Citation Subset:  IM; X    
Affiliation:
Coulter Corporation, Miami, FL 33196-2500, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / metabolism
Antibody Specificity
Antigens, CD4 / immunology
Antigens, CD8 / immunology
CD4-Positive T-Lymphocytes / cytology*,  immunology,  metabolism
CD8-Positive T-Lymphocytes / cytology*,  immunology,  metabolism
Humans
Immunomagnetic Separation / methods*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD4; 0/Antigens, CD8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mapping of epitopes recognized by PM/Scl autoantibodies with gene-fragment phage display libraries.
Next Document:  A nitric oxide production bioassay for interferon-gamma.