Document Detail

A simple and rapid method for removal of specific cell populations from whole blood.
MedLine Citation:
PMID:  8946015     Owner:  NLM     Status:  MEDLINE    
A method is presented in which specific cells can be rapidly separated from whole blood by the use of antibody labelled dense particles utilizing gravity as a means of separation. This method specifically removes the targeted cells with minimal non-specific cell loss. The method is simple to perform and rapid, requiring less than 10 min for cell/particle binding and separation. The method is robust so that exact ratios of particles to cells are not needed. This method could be used in any separation scheme to remove specific cell populations without significant modification.
R K Zwerner; R J Schmittling; T R Russell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  198     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-01-07     Completed Date:  1997-01-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-202     Citation Subset:  IM; X    
Coulter Corporation, Miami, FL 33196-2500, USA.
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MeSH Terms
Antibodies, Monoclonal / metabolism
Antibody Specificity
Antigens, CD4 / immunology
Antigens, CD8 / immunology
CD4-Positive T-Lymphocytes / cytology*,  immunology,  metabolism
CD8-Positive T-Lymphocytes / cytology*,  immunology,  metabolism
Immunomagnetic Separation / methods*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD4; 0/Antigens, CD8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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