| A simple, rapid method for differential cell counts in porcine mammary secretions. | |
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MedLine Citation:
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PMID: 1781144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of the fluorescent dye acridine orange for making differential cell counts in mammary secretions from sows was investigated, and the variations in cell type during the lactation period were also studied. In untreated samples of mammary secretions polymorphonuclear leucocytes, mononuclear phagocytes, lymphocytes and epithelial cells could be identified with certainty, and the methodological error was small (1.80 to 2.22 per cent). The mammary secretions could be stored at 4 degrees C for six hours without any effect on the differential count. Washing the secretions decreased the percentage of polymorphonuclear leucocytes and increased the percentage of epithelial cells. The polymorphonuclear leucocytes predominated in colostrum (58.0 to 65.5 per cent) and epithelial cells predominated in milk (60 to 89 per cent). Polymorphonuclear leucocytes were the predominant phagocytes in all mammary secretions (7.6 to 65.5 per cent). |
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Authors:
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U Magnusson; H Rodriguez-Martinez; S Einarsson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Veterinary record Volume: 129 ISSN: 0042-4900 ISO Abbreviation: Vet. Rec. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-03-06 Completed Date: 1992-03-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0031164 Medline TA: Vet Rec Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 485-90 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynaecology, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange
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diagnostic use Animals Cell Count / methods, veterinary* Colostrum / cytology Epithelial Cells Female Leukocyte Count / methods, veterinary Lymphocytes / ultrastructure Mammary Glands, Animal / cytology, secretion* Microscopy, Electron / veterinary Milk / cytology Neutrophils / ultrastructure Phagocytes / ultrastructure Swine* |
| Chemical | |
Reg. No./Substance:
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65-61-2/Acridine Orange |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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