Document Detail


A simple, rapid method for differential cell counts in porcine mammary secretions.
MedLine Citation:
PMID:  1781144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of the fluorescent dye acridine orange for making differential cell counts in mammary secretions from sows was investigated, and the variations in cell type during the lactation period were also studied. In untreated samples of mammary secretions polymorphonuclear leucocytes, mononuclear phagocytes, lymphocytes and epithelial cells could be identified with certainty, and the methodological error was small (1.80 to 2.22 per cent). The mammary secretions could be stored at 4 degrees C for six hours without any effect on the differential count. Washing the secretions decreased the percentage of polymorphonuclear leucocytes and increased the percentage of epithelial cells. The polymorphonuclear leucocytes predominated in colostrum (58.0 to 65.5 per cent) and epithelial cells predominated in milk (60 to 89 per cent). Polymorphonuclear leucocytes were the predominant phagocytes in all mammary secretions (7.6 to 65.5 per cent).
Authors:
U Magnusson; H Rodriguez-Martinez; S Einarsson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Veterinary record     Volume:  129     ISSN:  0042-4900     ISO Abbreviation:  Vet. Rec.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-03-06     Completed Date:  1992-03-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0031164     Medline TA:  Vet Rec     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  485-90     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
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MeSH Terms
Descriptor/Qualifier:
Acridine Orange / diagnostic use
Animals
Cell Count / methods,  veterinary*
Colostrum / cytology
Epithelial Cells
Female
Leukocyte Count / methods,  veterinary
Lymphocytes / ultrastructure
Mammary Glands, Animal / cytology,  secretion*
Microscopy, Electron / veterinary
Milk / cytology
Neutrophils / ultrastructure
Phagocytes / ultrastructure
Swine*
Chemical
Reg. No./Substance:
65-61-2/Acridine Orange

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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