| A simple, rapid method for the determination of glucose, lactate, pyruvate, alanine, 3-hydroxybutyrate and acetoacetate on a single 20-mul blood sample. | |
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MedLine Citation:
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PMID: 7105409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Existing methods for the estimation of glucose, lactate, pyruvate, alanine, 3-hydroxybutyrate and acetoacetate normally require relatively large volumes of blood. In addition spectrophotometric methods are tedious and time-consuming. A series of simple methods for the measurement of these metabolites whereby all can be measured on a single 20-microl blood sample is described here. All methods, with the exception of that for glucose, which is measured spectrophotometrically, depend on the use of fluorimetry to determine the concentration of the reduced co-enzyme NADH. As far as is possible, the methods have been standardised for the sake of simplicity and convenience. In routine use they have proved to be both rapid and reliable. |
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Authors:
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R J Maughan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 122 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-10-29 Completed Date: 1982-10-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 231-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxybutyric Acid Acetoacetates* Alanine / blood* Blood Glucose / analysis* Fluorometry Humans Hydroxybutyrates / blood* Keto Acids / blood* Lactates / blood* Lactic Acid Methods Microchemistry NAD / analysis Physical Exertion Pyruvates / blood* Pyruvic Acid Spectrophotometry Starvation / blood |
| Chemical | |
Reg. No./Substance:
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0/Acetoacetates; 0/Blood Glucose; 0/Hydroxybutyrates; 0/Keto Acids; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 300-85-6/3-Hydroxybutyric Acid; 50-21-5/Lactic Acid; 53-84-9/NAD; 541-50-4/acetoacetic acid; 56-41-7/Alanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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