| A simple and rapid determination of biapenem in plasma by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 16875889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, rapid and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine biapenem concentrations in human plasma. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes, and biapenem was stabilized by immediate mixing the plasma with 1M 3-morpholinopropanesulfonic acid (MOPS) buffer (pH 7.0) (1:1). Biapenem was detected by ultraviolet absorbance at 300nm with no interfering plasma peak. The calibration curve of biapenem in human plasma was linear from 0.04 to 50microg/mL. The limit of detection was 0.01microg/mL, which was more than 40-fold lower than that of conventional plasma protein precipitation using ammonium sulfate. The assay has been clinically applied to pharmacokinetic studies in patients. |
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Authors:
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Kayo Ikeda; Kazuro Ikawa; Aki Ikeda; Yoshimi Nishikawa; Norifumi Morikawa |
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Publication Detail:
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Type: Journal Article; Validation Studies Date: 2006-07-27 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 844 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-13 Completed Date: 2007-01-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 148-52 Citation Subset: IM |
Affiliation:
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Department of Clinical Pharmacotherapy, Division of Clinical Pharmacotherapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. ikeda@hiroshima-u.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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blood*,
pharmacokinetics Chromatography, High Pressure Liquid / methods* Humans Reference Standards Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Thienamycins / blood*, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Thienamycins; 120410-24-4/biapenem |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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