Document Detail

A simple radioimmunoassay for estriol 3-sulfate in pregnancy plasma without deconjugation.
MedLine Citation:
PMID:  3837408     Owner:  NLM     Status:  MEDLINE    
A highly specific anti-estriol 3-sulfate antiserum was treated with 50% ammonium sulfate, and the crude globulin fraction was coupled to CNBr-activated Sepharose-4B. Addition of 0.1M Tris-HC1 buffer (pH 8.3) containing 0.1M glutamine to the solution of antigen-antibody enabled assaying without solvent-extraction or chromatography to remove endogenous interference. Subsequently, a direct radioimmunoassay using [6,7-3H]-estriol 3-sulfate as a radioactive ligand without deconjugation has been established and applied to the determination of estriol 3-sulfate levels in pregnancy plasma. The increasing plasma levels of estriol 3-sulfate are correlated with estriol levels over the period of gestation. The mean values of sulfated estriol concentration (A) in late pregnancy plasma were approximately 7 times as high as unconjugated estriol (B), but individual ratios of A to B showed considerable variability.
T Tanaka; N Suguro; A Kubodera
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  46     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1986-09-16     Completed Date:  1986-09-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  649-57     Citation Subset:  IM    
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MeSH Terms
Estriol / analogs & derivatives*,  blood
Radioimmunoassay / methods*
Time Factors
Reg. No./Substance:
481-95-8/estriol 3-sulfate; 50-27-1/Estriol

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