| A simple procedure for the isolation of schistosomules for biochemical studies or culture. | |
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MedLine Citation:
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PMID: 2617043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of cercariae of Schistosoma mansoni in a 1:1 mixture of Eagle's Minimal Essential Medium and rat serum at 42 degrees C for only 5 minutes, plus subsequent vortexing, resulted in at least 98% conversion of cercariae to schistosomules. Subsequent centrifugation before or after settling for 30 minutes in an ice bath, resulted in schistosomule preparations with about 20% or 10% contamination with tail segments, respectively. |
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Authors:
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P O Ogbunude; A Selim; A Haq; H P Baer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ) Volume: 40 ISSN: 0177-2392 ISO Abbreviation: Trop. Med. Parasitol. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1990-03-15 Completed Date: 1990-03-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8503728 Medline TA: Trop Med Parasitol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 337-8 Citation Subset: IM |
Affiliation:
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Department of Biological and Medical Research, King Faisal Specialist Hospital, Riyadh, Saudi Arabia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Centrifugation Culture Media Parasitology / methods Schistosoma mansoni / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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