Document Detail

A simple precipitation method for measuring sialic acid in apolipoprotein B-containing lipoproteins in plasma.
MedLine Citation:
PMID:  15989730     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: A convenient method for the measurement of sialic acid in plasma apoB-containing lipoproteins is described. METHODS: Dextran sulphate-Mg(2+) precipitation and enzymatic sialic acid assay were combined and applied to analysis of plasma from 96 healthy controls and 136 hyperlipidaemic subjects of types IIa (n=46), IIb (n=43), and IV (n=47). RESULTS: The sialic acid concentrations (mean+/-SD) in plasma apoB-containing lipoproteins were 19.4+/-5.9, 24.3+/-4.7 (P<0.0001 versus normal), 23.0+/-4.7 (P<0.0001), 27.9+/-5.2 (P<0.0001), and 22.3+/-3.4 mg/L (P<0.002), for normal, all types of hyperlipidaemia, types IIa, IIb, and IV, respectively. The contents of sialic acid in apoB were 2.03+/-0.41%, 2.09+/-0.35% (no significance versus normal), 1.86+/-0.27% (P<0.0001), 1.97+/-0.26% (P<0.02), and 2.28+/-0.41% (P<0.002), for normal, all types of hyperlipidaemia, types IIa, IIb, and IV, respectively. CONCLUSION: The content of sialic acid in apoB decreased significantly in type IIa but increased in type IV hyperlipidaemia, which may reflect the presence of sialic acid in very low-density lipoprotein apolipoproteins other than apoB. This simple precipitation method will be useful to evaluate the sialic acid content in low-density lipoprotein in hyperlipidaemic subjects, especially of type IIa.
Koji Shishino; Yuki Yoshioka; Kaori Hashimoto; Masaaki Ochi; Kenji Aono; Mitsuharu Murase; Haruhiko Osawa; Hideichi Makino; Shuichi Saheki
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Annals of clinical biochemistry     Volume:  42     ISSN:  0004-5632     ISO Abbreviation:  Ann. Clin. Biochem.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-01     Completed Date:  2005-08-30     Revised Date:  2010-03-23    
Medline Journal Info:
Nlm Unique ID:  0324055     Medline TA:  Ann Clin Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  298-300     Citation Subset:  IM    
Central Laboratory Department, Ehime University School of Medicine, Toon City, 791-0295, Ehime Prefecture, Japan.
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MeSH Terms
Apolipoproteins B / chemistry*
Chemical Precipitation
Hyperlipidemias / blood
Middle Aged
N-Acetylneuraminic Acid / analysis*,  blood*
Sensitivity and Specificity
Reg. No./Substance:
0/Apolipoproteins B; 131-48-6/N-Acetylneuraminic Acid

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