Document Detail


A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells.
MedLine Citation:
PMID:  1938466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple technique is described for ultrastructural enzyme cytochemistry using small volumes and numbers of single cells. Small aliquots (1-5 microliters) of single cells suspended in a glutaraldehyde fixative are incubated on poly-L-lysine-coated glass coverslips. Cells in contact with the coated coverslip become immobilized to that support. All changes of solutions are accomplished without repeated centrifugation.
Authors:
J M Robinson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Histochemical journal     Volume:  23     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-12-10     Completed Date:  1991-12-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  10-2     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210.
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MeSH Terms
Descriptor/Qualifier:
5'-Nucleotidase / analysis*
Acid Phosphatase / analysis
Animals
Cell Line / enzymology
Histocytological Preparation Techniques
Humans
Mice
Monocytes / enzymology*,  ultrastructure
Polylysine
Grant Support
ID/Acronym/Agency:
AI 24321/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
25104-18-1/Polylysine; EC 3.1.3.2/Acid Phosphatase; EC 3.1.3.5/5'-Nucleotidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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