| A simple method to diagnose adrenoleukodystrophy using a dried blood spot on filter paper. | |
| | |
MedLine Citation:
|
PMID: 3757269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new, simple method for the diagnosis of adrenoleukodystrophy (ALD) using a dried blood spot sample, is described. Fatty acid from the dried blood spot was extracted and methylated simultaneously with HCl-methanol. Fatty acid methyl esters were analyzed by gas chromatography-mass spectrometry. Fatty acid compositions of the blood spot from four patients with ALD and five healthy controls were determined from the mass chromatograms of the m/z 143 ion, [(CH2)6 COOCH3]+. The ratios of tetracosanoic acid to docosanoic acid (C24:0/C22:0) and hexacosanoic acid to docosanoic acid (C26:0/C22:0) were significantly greater in ALD patients than in the controls. The fatty acid composition of the dried blood spot did not change at room temperature within a week. Since the specimens can be sent by mail, this method could be applied to the screening of ALD. |
| | |
Authors:
|
H Nishio; S Kodama; S Yokoyama; T Matsuo; T Mio; K Sumino |
Related Documents
:
|
20152459 - Evaluation of a new electrolytic cold vapor generation system for mercury determination... 21446699 - Veraguamides a-g, cyclic hexadepsipeptides from a dolastatin 16-producing cyanobacteriu... 9338989 - Automated polarographic determination of sulfide as contaminant in parenteral amino aci... 954179 - Effect of different lysing and washing methods on free amino acid concentrations of she... 18962949 - The separation and determination of fatty acids by isotopic dilution and radiogas-liqui... 11671989 - Acid-base equilibria in nonpolar media. 1. a spectrophotometric method for acidity meas... 6745419 - Fucosyl gangliosides of pc12 pheochromocytoma cells. 11442389 - Solid-phase directed ortho-lithiation and the preparation of a phthalide library. 9478889 - Carbonic acid in the gas phase and its astrophysical relevance |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinica chimica acta; international journal of clinical chemistry Volume: 159 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1986 Aug |
Date Detail:
|
Created Date: 1986-11-14 Completed Date: 1986-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 77-82 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenoleukodystrophy
/
blood,
diagnosis* Diffuse Cerebral Sclerosis of Schilder / diagnosis* Fatty Acids / blood Filtration / instrumentation Gas Chromatography-Mass Spectrometry Humans Specimen Handling Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The nature of gastric secretions in a patient with Zollinger-Ellison syndrome without peptic ulcerat...
Next Document: Epidemiology of primary hypertension in the young.