Document Detail


A simple method for the production of anti-C3d monoclonal antibody.
MedLine Citation:
PMID:  18158789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Production of monoclonal antibodies to C3d usually involves the purification of protein. Our method does not require C3 purification; it relies on attachment of C3b to mouse erythrocytes by activation of alternative pathways and further conversion in C3d. We prepared human complement-coated mouse red cells and sensitized mice of the same strain with our own schedule of immunization and applied the classical methods to obtain a mouse monoclonal antibody. We obtained a clone called BMS-11 which produces a monoclonal antibody of IgM class, to C3d with a title of 1:500000. The monoclonal antibody obtained has shown that it is suitable for use as an antiglobulin reagent.
Authors:
Carlos Cruz; Graciela León
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hybridoma (2005)     Volume:  26     ISSN:  1554-0014     ISO Abbreviation:  Hybridoma (Larchmt)     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-26     Completed Date:  2008-03-31     Revised Date:  2008-05-28    
Medline Journal Info:
Nlm Unique ID:  101241539     Medline TA:  Hybridoma (Larchmt)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  433-4     Citation Subset:  IM    
Affiliation:
Banco M de Sangre del DC, Immunohematología, Caracas, Venezuela.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis*
Ascitic Fluid / immunology
Carcinoma, Ehrlich Tumor / immunology
Complement C3b / metabolism
Complement C3d / biosynthesis,  immunology*
Humans
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 80295-43-8/Complement C3b; 80295-45-0/Complement C3d

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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