Document Detail


A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability.
MedLine Citation:
PMID:  2374136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse morulae were exposed to solutions containing 30-50% of permeable agents (ethylene glycol, glycerol, propylene glycol) in modified phosphate-buffered saline (PB1 medium) at 20 degrees C for 20 min. A high percentage of them developed to expanded blastocysts in culture, after exposure to 30% and 40% ethylene glycol (98 and 84%, respectively), or 30% glycerol (88%). Ethylene glycol and glycerol were diluted to 30 and 40% with PB1 medium or with PB1 containing 30% Ficoll or 30% Ficoll + 0.5 M-sucrose, immersed in liquid nitrogen in straws and warmed in 20 degrees C water. Solutions containing 40% of a permeable agent with Ficoll did not crystallize during cooling or warming. Mouse morulae were exposed to 40% ethylene glycol in PB1 medium containing 30% Ficoll (EF) or PB1 medium + 30% Ficoll + 0.5 M-sucrose (EFS) for 5-20 min at 20 degrees C. EFS solution was non-toxic to the embryos during 5 min of exposure. When embryos, equilibrated in EFS solution for 2 or 5 min at 20 degrees C, were vitrified at -196 degrees C and were warmed rapidly, nearly all embryos developed in culture (97-98%), and 51% developed to live young at term after transfer. This method, which results in virtually no decrease in embryonic viability, may be of practical use for embryo preservation.
Authors:
M Kasai; J H Komi; A Takakamo; H Tsudera; T Sakurai; T Machida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  89     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-08-30     Completed Date:  1990-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  91-7     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Science, College of Agriculture, Kochi University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cryopreservation / methods*
Cryoprotective Agents
Culture Media
Embryo Transfer*
Ethylene Glycol
Ethylene Glycols
Female
Ficoll
Glycerol
Mice
Mice, Inbred ICR
Propylene Glycol
Propylene Glycols
Sucrose
Tissue Preservation / methods*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media; 0/Ethylene Glycols; 0/Propylene Glycols; 107-21-1/Ethylene Glycol; 25702-74-3/Ficoll; 56-81-5/Glycerol; 57-50-1/Sucrose; 57-55-6/Propylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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