Document Detail


A simple method for the measurement of the steroid fraction bound to sex hormone binding globulin in serum.
MedLine Citation:
PMID:  2770298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The steroid-protein binding equilibrium in extra-cellular fluids is considered to have possible relevance to the development of endometrial and breast cancer. A method is described to measure the fraction of steroid bound to sex hormone binding globulin (SHBG) in serum, using binding of SHBG to Con-A-sepharose. To validate the assay, serum samples with different SHBG levels were studied at various dilutions and with various amounts of added ligands. Sera with high SHBG concentrations still showed considerable steroid binding after heating to 59 degrees C for 30 min. The intra-assay variation of the assay ranged from 2-4%, the inter-assay variation was approx. 5%.
Authors:
J M Bonfrer; P F Bruning; W J Nooijen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  33     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-29     Completed Date:  1989-09-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  227-31     Citation Subset:  IM    
Affiliation:
The Netherlands Cancer Institute, Division of Clinical Oncology, Amsterdam.
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MeSH Terms
Descriptor/Qualifier:
Binding, Competitive
Estradiol / blood
Female
Humans
Male
Pregnancy
Sex Hormone-Binding Globulin / metabolism*
Steroids / blood*
Testosterone / blood
Chemical
Reg. No./Substance:
0/Sex Hormone-Binding Globulin; 0/Steroids; 50-28-2/Estradiol; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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