Document Detail

A simple method for mass-production of liposomes, in particular large liposomes, suitable for delivery of free amino acids to filter feeding zooplankton.
MedLine Citation:
PMID:  17613698     Owner:  NLM     Status:  MEDLINE    
In marine fish larviculture the live feed organisms are often enriched in order to enhance their nutritional value. One of the challenges is to enhance the phospholipids (PL) content, and another is to enhance the content of specific water soluble nutrients, like free amino acids (FAA). There are a few studies where this has been achieved by the use of liposomes. The aim of this study was to develop a simple method for mass-production of liposomes within a size range of 1-5 mum a size range suitable to feed live food organisms. Furthermore, the liposomes should have a high FAA concentration and be stable under conditions typical for short-time enrichment of live feed organisms. The method used in the present study is based on a combination of a reverse-phase evaporation method for preparing liposomes and re-hydration of freeze-dried, empty liposomes. The liposomal membrane was made of soy phosphatydilcholine and was loaded with a highly concentrated free amino acids solution. Most of the liposomes produced were 2-8 mum in diameter and the FAA encapsulation efficiency was 42.6%. Two experiments simulating 2 hr of live food enrichment were used to evaluate the liposomes. The results showed the liposome did not disintegrate or aggregate when suspended in seawater and that only 9% of the FAA content of the liposomes was lost after 2 hr suspension. The developed method was easy and reliable, producing tens of grams of liposomes per batch.
Yoav Barr; Synnøve Helland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of liposome research     Volume:  17     ISSN:  0898-2104     ISO Abbreviation:  J Liposome Res     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-06     Completed Date:  2007-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001952     Medline TA:  J Liposome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-88     Citation Subset:  IM    
AKVAFORSK, Institute of Aquaculture Research AS, Sunndalsøra, Norway.
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MeSH Terms
Amino Acids / chemistry,  pharmacology*
Animal Feed*
Liposomes / chemistry,  pharmacology*
Phospholipids / chemistry,  pharmacology
Zooplankton / growth & development*
Reg. No./Substance:
0/Amino Acids; 0/Liposomes; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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