Document Detail

A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy.
MedLine Citation:
PMID:  9328584     Owner:  NLM     Status:  MEDLINE    
In this report we describe a simple method using the vital dye 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE) to follow splenic graft rejection by flow cytometry. CFSE-labelled spleen cell suspensions were injected intravenously into various recipients and blood samples were taken at different time points to follow the transferred cells. We found that the labelled cells could be readily detected by flow cytometry for up to eleven weeks. The loss of these labelled cells in various transfusion experiments with different major and minor histocompatibility differences followed the rejection kinetics previously described for skin transplants. Thus, this method offers a simple tool to test the histocompatibility of donor cells/grafts with the host in adoptive/transplantation experiments in which donor and host are not completely syngeneic. Furthermore, we developed a method to trace adoptively transferred fluorescent CFSE-labelled cells by light microscopy by converting in situ immunofluorescence staining into immunoenzyme staining.
S Oehen; K Brduscha-Riem; A Oxenius; B Odermatt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunological methods     Volume:  207     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-31     Completed Date:  1997-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  33-42     Citation Subset:  IM    
Institut für Experimentelle Immunologie, University Hospital Zürich, Switzerland.
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MeSH Terms
Adoptive Transfer
Amino Acid Sequence
Cell Transplantation*
Cells, Cultured
Flow Cytometry / methods*
Graft Rejection / diagnosis*,  immunology
Immunohistochemistry / methods
Lymphocyte Activation
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Spleen / cytology*
T-Lymphocytes / immunology
Reg. No./Substance:
0/5-(6)-carboxyfluorescein diacetate succinimidyl ester; 0/Fluoresceins; 0/Succinimides

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