| A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler. | |
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MedLine Citation:
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PMID: 15816417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98. |
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Authors:
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S Uday Bhaskar; G Gopalaswamy; R Raghu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Indian journal of experimental biology Volume: 43 ISSN: 0019-5189 ISO Abbreviation: Indian J. Exp. Biol. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-04-08 Completed Date: 2005-05-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0233411 Medline TA: Indian J Exp Biol Country: India |
Other Details:
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Languages: eng Pagination: 277-9 Citation Subset: IM |
Affiliation:
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Department of Plant Molecular Biology and Biotechnology, Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Red
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metabolism Ammonium Sulfate / pharmacology Bacterial Proteins / metabolism* Biochemistry / methods* Chromatography, Gel Cyanobacteria / metabolism Electrophoresis, Polyacrylamide Gel Light Nitrogen Phycocyanin / chemistry*, isolation & purification* Plant Extracts Spectrophotometry Spirulina |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Plant Extracts; 11016-15-2/Phycocyanin; 7727-37-9/Nitrogen; 7783-20-2/Ammonium Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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