Document Detail


A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler.
MedLine Citation:
PMID:  15816417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98.
Authors:
S Uday Bhaskar; G Gopalaswamy; R Raghu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  43     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-04-08     Completed Date:  2005-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  India    
Other Details:
Languages:  eng     Pagination:  277-9     Citation Subset:  IM    
Affiliation:
Department of Plant Molecular Biology and Biotechnology, Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, India.
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MeSH Terms
Descriptor/Qualifier:
Algae, Red / metabolism
Ammonium Sulfate / pharmacology
Bacterial Proteins / metabolism*
Biochemistry / methods*
Chromatography, Gel
Cyanobacteria / metabolism
Electrophoresis, Polyacrylamide Gel
Light
Nitrogen
Phycocyanin / chemistry*,  isolation & purification*
Plant Extracts
Spectrophotometry
Spirulina
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Plant Extracts; 11016-15-2/Phycocyanin; 7727-37-9/Nitrogen; 7783-20-2/Ammonium Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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