| A simple method for the determination of lipid composition of human bile. | |
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MedLine Citation:
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PMID: 2066669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Entero-Test, a device for easy sampling of gastrointestinal contents, including bile, has been used for determination of biliary lipid composition. The device consists of a weighted gelatin capsule containing 140 cm of a highly absorbent nylon line. The capsule is swallowed while one end of the string is taped to the face. After 3.5 h, when the line has reached the duodenum, gallbladder contraction is stimulated by intramuscular administration of ceruletide. The line is pulled out, and the last 15 cm are eluted four times in methanol. Total bile acids (by 3 alpha-hydroxysteroid-dehydrogenase assay), individual bile acids (by high performance liquid chromatography), phospholipids (by assay of lipid-soluble phosphorus), and cholesterol (by gas-liquid chromatography) are determined in the eluate. Tests in vitro demonstrated no preferential binding and a good recovery of biliary lipids from the thread. Similar values of biliary cholesterol saturation were obtained by means of duodenal intubation and of the Entero-Test in a series of 12 subjects (r = 0.952). In 5 subjects, individual bile acids were also measured and were found to be similar with both techniques (r = 0.948). When the test was repeated over 3 days in a series of 7 subjects, biliary cholesterol saturation was found to be remarkably reproducible (CV = 7.6%). Thus, the Entero-Test is a convenient technique for the determination of biliary lipid composition, which can be particularly useful in longitudinal studies. |
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Authors:
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M Muraca; M T Vilei; L Miconi; P Petrin; M Antoniutti; S Pedrazzoli |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of lipid research Volume: 32 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-08-12 Completed Date: 1991-08-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 371-4 Citation Subset: IM |
Affiliation:
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Istituto di Medicina Interna Cattedra di Patologia Chirurgica, University of Padua, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bile
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chemistry* Bile Acids and Salts / analysis Caerulein / administration & dosage Cholesterol / analysis Duodenum / chemistry* Humans Lipids / analysis* Methods Phospholipids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Lipids; 0/Phospholipids; 17650-98-5/Caerulein; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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