Document Detail

A simple method for cryopreservation of MDBK cells using trehalose and storage at -80 degrees C.
MedLine Citation:
PMID:  19381873     Owner:  NLM     Status:  MEDLINE    
Madin Darby bovine kidney cells were stored at -80 degrees C using trehalose. Trehalose was loaded into the cells by fluid-phase endocytosis that was facilitated by heat shock at 40 degrees C for 1 h. Loaded cells were gradually frozen and stored at -80 degrees C. Revival of cells was done by quick thawing and immediately seeded in the tissue culture flasks. The membrane integrity of cells was measured at different times post-storage by trypan blue dye exclusion method. It was estimated to be 96.23, 73.84, 57.33, 54.36, 25.47, 50.53 and 46.86% at 0, 7, 60, 90, 120, 160 and 180-day post-storage, respectively. Cryostorage of cells at -80 degrees C may help to reduce the use of liquid nitrogen.
Bimalendu Mondal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-18
Journal Detail:
Title:  Cell and tissue banking     Volume:  10     ISSN:  1573-6814     ISO Abbreviation:  Cell Tissue Bank     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2010-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965121     Medline TA:  Cell Tissue Bank     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  341-4     Citation Subset:  IM    
Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital, 263138, Uttarakhand, India.
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MeSH Terms
Cell Line
Cryopreservation / methods*
Cryoprotective Agents / metabolism
Hot Temperature
Kidney / cytology*
Trehalose / metabolism*
Reg. No./Substance:
0/Cryoprotective Agents; 99-20-7/Trehalose

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