Document Detail


A simple method for cloning and replica plating of mammalian cells using multicellular spheroids.
MedLine Citation:
PMID:  356496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
V 79/4 Chinese hamster cells or HeLa cells grow in Eagle's MEM supplemented with 25 microgram/ml dextran sulphate to form clonal multicellular spheroids. These cell clones, consisting of 5-10(2) cells, are easy to separate, to transfer from one culture vessel into another and grow as normal monolayer colonies on Dederon cloth circles after subculture in Eagle's MEM without dextran sulphate. A simple replica technique is described by which 500 clones can be transfered onto at least 3 replica cloth circles, 10 cm in diameter, with a replica plating efficiency of approximately 100%.
Authors:
W Handschack; J Kopp; C Pitra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biologica et medica Germanica     Volume:  36     ISSN:  0001-5318     ISO Abbreviation:  Acta Biol. Med. Ger.     Publication Date:  1977  
Date Detail:
Created Date:  1978-10-27     Completed Date:  1978-10-27     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370276     Medline TA:  Acta Biol Med Ger     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  1429-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Clone Cells / cytology*
Cricetinae
Cricetulus
Culture Media
Cytological Techniques
Fibroblasts / cytology
Hela Cells / cytology
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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