Document Detail


A simple lavage method for the cytologic sampling of DMBA-induced carcinomas of the hamster cheek pouch.
MedLine Citation:
PMID:  6812336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An effective, simple sampling device was developed to obtain improved cytologic specimens from the cheek pouches of hamsters; it was tested in the sampling of squamous-cell carcinomas induced with 9,10-dimethyl-1,2-benzanthracene (DMBA). Cellular samples collected by this modified syringe lavage-aspirator using Hanks' balanced salt solution contained an average of 1.3 X 10(5) cells per milliliter. Of the heterogeneous contents of the cheek pouch (food, debris, erythrocytes, leukocytes, macrophages, squamous cells, atypical cells and cancer cells), the cells of diagnostic significance from cancer and dysplasia were increased by this procedure to 3.7% of the cells recovered, as compared to 2.5% of the cells obtained by direct scraping of the same pouches. The inexpensive, disposable device allowed repeated sampling of cheek pouches without causing injury to the animals.
Authors:
C D Albright; D P Colvin; J K Frost; B R Marsh; D H Hopp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cytologica     Volume:  26     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    1982 Jul-Aug
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  542-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
9,10-Dimethyl-1,2-benzanthracene
Animals
Cheek
Cricetinae
Irrigation / methods*
Male
Mesocricetus
Mouth Mucosa / pathology*
Mouth Neoplasms / chemically induced,  pathology*
Neoplasms, Experimental / chemically induced,  pathology
Chemical
Reg. No./Substance:
57-97-6/9,10-Dimethyl-1,2-benzanthracene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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