Document Detail


A simple in vivo bioassay for inhibin-like activity using ovariectomized ewes.
MedLine Citation:
PMID:  3149225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Long term ovariectomized ewes were used in a bioassay for inhibin-like activity. The concentration of FSH 6 to 7 hr after injection of follicular fluid (a rich source of inhibin), as a percentage of pretreatment, regressed on the log of the dose had a slope of -26.0 +/- 7.6 (5 replications, mean +/- SD) and an index of precision of .32 +/- .04. This system was rapid, relatively easy and specific for in vivo inhibin-like activity. This bioassay was also used to determine the relative potency of an affinity-purified fraction of follicular fluid.
Authors:
K F Miller; D J Bolt
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  4     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1989-05-18     Completed Date:  1989-05-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods
Body Fluids / physiology
Cattle
Female
Follicle Stimulating Hormone / analysis*
Inhibins / analysis*
Ovarian Follicle / physiology
Ovariectomy / veterinary
Sheep / physiology*
Chemical
Reg. No./Substance:
57285-09-3/Inhibins; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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