Document Detail


A simple freezing and storage method to preserve the stimulating function of leukocytes in the mixed leukocyte reaction.
MedLine Citation:
PMID:  125468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human peripheral blood leukocytes retain their capacity to function as stimulating cells in the one way MLR after freezing and storage at - 70 degrees C by a simple and inexpensive method. This storage method facilitates the use of a standard stimulating pool of cells from three donors which may provide a quantitative assay for testing the ability of cells to respond to allogeneic cells in culture.
Authors:
D Osoba; J Falk; M Dajko; J Richardson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue antigens     Volume:  5     ISSN:  0001-2815     ISO Abbreviation:  Tissue Antigens     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-11-06     Completed Date:  1975-11-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0331072     Medline TA:  Tissue Antigens     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  226-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Freezing*
Humans
Lymphocyte Activation
Lymphocyte Culture Test, Mixed*
Time Factors
Tissue Preservation / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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