| A simple fractionation of chicken egg yolk yields a protein component that stimulates cell proliferation and differentiation in primary avian tendon cells. | |
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MedLine Citation:
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PMID: 3710995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An easily prepared and stable protein fraction from chick egg yolk promotes cell division (40 h generation time) and expression of procollagen (60% of total protein synthesis) in primary avian tendon cells in a serum-free medium. The activity of this yolk fraction (YF) is proteinaceous as reflected by its sensitivity to protease treatment. Yolk fraction is resolved into four major components on sodium dodecyl sulfate polyacrylamide gel electrophoresis with apparent molecular weights of 82,70,42,35 (X 10(-3)). Under nondenaturing conditions, YF runs as a mixture of high molecular weight aggregates on Sephacryl G-200. We postulate that the active part of YF could be the in ovo growth promoter for embryonic chick tendon cells. |
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Authors:
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M J Martis; R I Schwarz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: In vitro cellular & developmental biology : journal of the Tissue Culture Association Volume: 22 ISSN: 0883-8364 ISO Abbreviation: In Vitro Cell. Dev. Biol. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-07-01 Completed Date: 1986-07-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8506951 Medline TA: In Vitro Cell Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 241-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / drug effects Cell Division / drug effects Cells, Cultured Chick Embryo Culture Media Egg Proteins / isolation & purification, pharmacology* Egg Yolk / analysis* Growth Substances / isolation & purification* Molecular Weight Procollagen / biosynthesis Tendons / cytology*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA 37958/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Egg Proteins; 0/Growth Substances; 0/Procollagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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