Document Detail


A simple fractionation of chicken egg yolk yields a protein component that stimulates cell proliferation and differentiation in primary avian tendon cells.
MedLine Citation:
PMID:  3710995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An easily prepared and stable protein fraction from chick egg yolk promotes cell division (40 h generation time) and expression of procollagen (60% of total protein synthesis) in primary avian tendon cells in a serum-free medium. The activity of this yolk fraction (YF) is proteinaceous as reflected by its sensitivity to protease treatment. Yolk fraction is resolved into four major components on sodium dodecyl sulfate polyacrylamide gel electrophoresis with apparent molecular weights of 82,70,42,35 (X 10(-3)). Under nondenaturing conditions, YF runs as a mixture of high molecular weight aggregates on Sephacryl G-200. We postulate that the active part of YF could be the in ovo growth promoter for embryonic chick tendon cells.
Authors:
M J Martis; R I Schwarz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  22     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-07-01     Completed Date:  1986-07-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  241-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects
Cell Division / drug effects
Cells, Cultured
Chick Embryo
Culture Media
Egg Proteins / isolation & purification,  pharmacology*
Egg Yolk / analysis*
Growth Substances / isolation & purification*
Molecular Weight
Procollagen / biosynthesis
Tendons / cytology*,  metabolism
Grant Support
ID/Acronym/Agency:
CA 37958/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Egg Proteins; 0/Growth Substances; 0/Procollagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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