| A simple flow injection spectrophotometric procedure for the determination of diazepam in pharmaceutical formulation. | |
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MedLine Citation:
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PMID: 16429787 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A single-channel flow injection (FI) manifold with spectrophotometric detection has been designed and fabricated for diazepam determination. A 100 microl sample and/or standard solution containing diazepam was injected into a flowing stream of 0.1 mol L(-1) hydrochloric acid with the optimum flow rate of 6.8 mL min(-1). As soon as the sample reached the detector, the FI signal as a peak was recorded at 360 nm. The optimum conditions for microg amounts of diazepam were achieved. A linear calibration graph over the range of 2-110 mg L(-1) diazepam was obtained with the regression equation Y = 0.2926X + 0.5896 (r2 = 0.9929). The method was very sensitive, since as little as 0.60 mg L(-1) could be detected; very reproducible with an RSD of 3.3% (n=11); and very rapid with a sampling rate of 100 h(-1). The limit of quantitation (10 sigma) was 2.0 mg L(-1). The proposed FI procedure has been satisfactorily applied to the quantitation of diazepam in commercial pharmaceutical formulations. The obtained results were in excellent agreement with those obtained by the conventional spectrophotometric method, verified by the student t-test at the 95% confidence level. |
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Authors:
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S Liawruangrath; Jirayu Makchit; B Liawruangrath |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 22 ISSN: 0910-6340 ISO Abbreviation: Anal Sci Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-24 Completed Date: 2006-04-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 127-30 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. scislwrn@chiangmai.ac.th |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Diazepam
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analysis* Equipment Design Flow Injection Analysis / instrumentation, methods Pharmaceutical Preparations / chemistry* Sensitivity and Specificity Spectrophotometry, Ultraviolet / instrumentation, methods |
| Chemical | |
Reg. No./Substance:
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0/Pharmaceutical Preparations; 439-14-5/Diazepam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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